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restriction enzyme digestion with bfuai  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction enzyme digestion with bfuai
    Restriction Enzyme Digestion With Bfuai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/restriction+enzyme+digestion+with+bfuai/BfuAI/pm42021122-118-15-20
    Average 95 stars, based on 75 article reviews
    restriction enzyme digestion with bfuai - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A synthetic DNA template for fast manufacturing of versatile single epitope mRNA.
    Article Snippet: Cloned plasmids were sequence verified (Eurofins Genomics), and selected clones were further amplified by MIDI DNA preparation using plasmid kits from Qiagen. .. Each plasmid was linearized overnight by restriction enzyme digestion with BfuAI (NEB) to enable in vitro mRNA transcription. .. As quality controls, the yield (absorbance at 260/280 nm), integrity (BioAnalyzer 2100, DNA 7500 chip), and sequence (Eurofins Genomics) of both SDT and PDT were verified. mRNA synthesis The iVT reaction was performed starting from a dsDNA template using a T7 enzyme mix containing: T7 RNA polymerase (Thermo Fisher Scientific), RNase inhibitor (Promega), and inorganic pyrophosphatase (Thermo Fisher Scientific).

    Article Title: An mRNA mix redirects dendritic cells towards an antiviral program, inducing anticancer cytotoxic stem cell and central memory CD8 + T cells
    Article Snippet: Cloned plasmids were sequence-verified (Eurofins Genomics) and selected clones were further amplified by MIDI DNA-preparation using QIAGEN Plasmid Kits – (QIAGEN). .. Each plasmid was linearized overnight by restriction enzyme digestion with BfuAI (NEB) to enable in vitro mRNA transcription. .. A thorough quality control was performed assessing the yield (absorbance at 260/280 nm), integrity (BioAnalyzer 2100, DNA 7500 chip) and sequence (Eurofins Genomics) of each plasmid.

    Article Title: An mRNA-based workflow validating neo-epitope presentation through HLA-I/peptide affinity purification
    Article Snippet: Cloned pLMCT-plasmids were screened based on the sequence specific restriction digestion pattern and were sequence verified (Eurofins Genomics). .. After amplification of selected bacterial clones, pLMCT-plasmids were isolated (Qiagen Midi Plasmid Kits) and linearized over night by restriction enzyme digestion with BfuAI (NEB). ..

    Article Title: A synthetic DNA template for fast manufacturing of versatile single epitope mRNA
    Article Snippet: Cloned plasmids were sequence verified (Eurofins Genomics), and selected clones were further amplified by MIDI DNA preparation using plasmid kits from Qiagen. .. Each plasmid was linearized overnight by restriction enzyme digestion with BfuAI (NEB) to enable in vitro mRNA transcription. .. As quality controls, the yield (absorbance at 260/280 nm), integrity (BioAnalyzer 2100, DNA 7500 chip), and sequence (Eurofins Genomics) of both SDT and PDT were verified.

    Article Title: Sequence simplification of antigen coding IVT mRNA allows accelerated synthetic DNA template generation and epitope immunogenicity validation
    Article Snippet: Selected clones were further amplified by MIDI DNA preparation using QIAGEN Plasmid Kits (QIAGEN). .. Each plasmid DNA was linearized overnight by restriction enzyme digestion with BfuAI (NEB) prior to in vitro mRNA transcription reaction. .. Plasmid DNA templates were designed, so enzymatic digestion with XhoI and SalI (NEB) cuts off the 3′ UTR and the poly-A tail, respectively.

    In Vitro:

    Article Title: A synthetic DNA template for fast manufacturing of versatile single epitope mRNA.
    Article Snippet: Cloned plasmids were sequence verified (Eurofins Genomics), and selected clones were further amplified by MIDI DNA preparation using plasmid kits from Qiagen. .. Each plasmid was linearized overnight by restriction enzyme digestion with BfuAI (NEB) to enable in vitro mRNA transcription. .. As quality controls, the yield (absorbance at 260/280 nm), integrity (BioAnalyzer 2100, DNA 7500 chip), and sequence (Eurofins Genomics) of both SDT and PDT were verified. mRNA synthesis The iVT reaction was performed starting from a dsDNA template using a T7 enzyme mix containing: T7 RNA polymerase (Thermo Fisher Scientific), RNase inhibitor (Promega), and inorganic pyrophosphatase (Thermo Fisher Scientific).

    Article Title: An mRNA mix redirects dendritic cells towards an antiviral program, inducing anticancer cytotoxic stem cell and central memory CD8 + T cells
    Article Snippet: Cloned plasmids were sequence-verified (Eurofins Genomics) and selected clones were further amplified by MIDI DNA-preparation using QIAGEN Plasmid Kits – (QIAGEN). .. Each plasmid was linearized overnight by restriction enzyme digestion with BfuAI (NEB) to enable in vitro mRNA transcription. .. A thorough quality control was performed assessing the yield (absorbance at 260/280 nm), integrity (BioAnalyzer 2100, DNA 7500 chip) and sequence (Eurofins Genomics) of each plasmid.

    Article Title: A synthetic DNA template for fast manufacturing of versatile single epitope mRNA
    Article Snippet: Cloned plasmids were sequence verified (Eurofins Genomics), and selected clones were further amplified by MIDI DNA preparation using plasmid kits from Qiagen. .. Each plasmid was linearized overnight by restriction enzyme digestion with BfuAI (NEB) to enable in vitro mRNA transcription. .. As quality controls, the yield (absorbance at 260/280 nm), integrity (BioAnalyzer 2100, DNA 7500 chip), and sequence (Eurofins Genomics) of both SDT and PDT were verified.

    Article Title: Sequence simplification of antigen coding IVT mRNA allows accelerated synthetic DNA template generation and epitope immunogenicity validation
    Article Snippet: Selected clones were further amplified by MIDI DNA preparation using QIAGEN Plasmid Kits (QIAGEN). .. Each plasmid DNA was linearized overnight by restriction enzyme digestion with BfuAI (NEB) prior to in vitro mRNA transcription reaction. .. Plasmid DNA templates were designed, so enzymatic digestion with XhoI and SalI (NEB) cuts off the 3′ UTR and the poly-A tail, respectively.

    Amplification:

    Article Title: An mRNA-based workflow validating neo-epitope presentation through HLA-I/peptide affinity purification
    Article Snippet: Cloned pLMCT-plasmids were screened based on the sequence specific restriction digestion pattern and were sequence verified (Eurofins Genomics). .. After amplification of selected bacterial clones, pLMCT-plasmids were isolated (Qiagen Midi Plasmid Kits) and linearized over night by restriction enzyme digestion with BfuAI (NEB). ..

    Article Title: Clathrin adaptor EPSIN1 (EPS1) modulates plasma membrane abundance of PLEIOTROPIC DRUG RESISTANCE PDR9 for effective hormone homeostasis.
    Article Snippet: Despite its key role at the root–soil interface, the cellularmachinery that directs plasma membrane (PM) proteins, such as the indole3-butyric acid (IBA) effluxer PLEIOTROPIC DRUG RESISTANCE 9 (PDR9), to the outer root epidermis remains poorly understood.. This study identified the clathrin adaptor EPSIN1 (EPS1), localized to the trans-Golgi Network/early endosome (TGN/EE), as a novel modulator of PDR9 PM abundance and IBA-dependent physiological processes, thereby expanding the limited understanding of biological roles of plant EPSIN family members.. In plants, the protein composition of the PM plays a critical role in mediating interactions between the cell and its environment, enabling cells to mount appropriate responses to biotic and abiotic stresses and processes related to growth and development.

    Clone Assay:

    Article Title: An mRNA-based workflow validating neo-epitope presentation through HLA-I/peptide affinity purification
    Article Snippet: Cloned pLMCT-plasmids were screened based on the sequence specific restriction digestion pattern and were sequence verified (Eurofins Genomics). .. After amplification of selected bacterial clones, pLMCT-plasmids were isolated (Qiagen Midi Plasmid Kits) and linearized over night by restriction enzyme digestion with BfuAI (NEB). ..

    Isolation:

    Article Title: An mRNA-based workflow validating neo-epitope presentation through HLA-I/peptide affinity purification
    Article Snippet: Cloned pLMCT-plasmids were screened based on the sequence specific restriction digestion pattern and were sequence verified (Eurofins Genomics). .. After amplification of selected bacterial clones, pLMCT-plasmids were isolated (Qiagen Midi Plasmid Kits) and linearized over night by restriction enzyme digestion with BfuAI (NEB). ..

    Polymerase Chain Reaction:

    Article Title: Clathrin adaptor EPSIN1 (EPS1) modulates plasma membrane abundance of PLEIOTROPIC DRUG RESISTANCE PDR9 for effective hormone homeostasis.
    Article Snippet: Despite its key role at the root–soil interface, the cellularmachinery that directs plasma membrane (PM) proteins, such as the indole3-butyric acid (IBA) effluxer PLEIOTROPIC DRUG RESISTANCE 9 (PDR9), to the outer root epidermis remains poorly understood.. This study identified the clathrin adaptor EPSIN1 (EPS1), localized to the trans-Golgi Network/early endosome (TGN/EE), as a novel modulator of PDR9 PM abundance and IBA-dependent physiological processes, thereby expanding the limited understanding of biological roles of plant EPSIN family members.. In plants, the protein composition of the PM plays a critical role in mediating interactions between the cell and its environment, enabling cells to mount appropriate responses to biotic and abiotic stresses and processes related to growth and development.

    Sequencing:

    Article Title: Clathrin adaptor EPSIN1 (EPS1) modulates plasma membrane abundance of PLEIOTROPIC DRUG RESISTANCE PDR9 for effective hormone homeostasis.
    Article Snippet: Despite its key role at the root–soil interface, the cellularmachinery that directs plasma membrane (PM) proteins, such as the indole3-butyric acid (IBA) effluxer PLEIOTROPIC DRUG RESISTANCE 9 (PDR9), to the outer root epidermis remains poorly understood.. This study identified the clathrin adaptor EPSIN1 (EPS1), localized to the trans-Golgi Network/early endosome (TGN/EE), as a novel modulator of PDR9 PM abundance and IBA-dependent physiological processes, thereby expanding the limited understanding of biological roles of plant EPSIN family members.. In plants, the protein composition of the PM plays a critical role in mediating interactions between the cell and its environment, enabling cells to mount appropriate responses to biotic and abiotic stresses and processes related to growth and development.



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